extraction buffer【7.8 mL TEN buffer, 2mL 20% SDS, 200 μL 0.8 M DTT, add sterile ddH2O or DEPC-ddH2O to 10 mL】
TEN buffer:Tris-Base 1.211g, EDTA0.74448 g, NaCl 1.1688g, add ddH2O to 100 mL and adjust pH to 8.0, and steriled at 121oC for 20-15 minutes.